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M., Tsai, H
and Paul, F
For an in-depth understanding, you can refer to studies from Grand View Research, Mordor Intelligence, and Statista linked below
Treatment of mutant lysates or supernatants with the reducing agent DTT (dithiothreitol) collapsed practically all the protein to monomeric size, suggesting that disulfide bonds, either intramolecular or intermolecular, are required for observation of multimers and larger species on denaturing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
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