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The enzyme was dialyzed by 20 kDa cut-off membrane (Cat#0.54600051) against polyethylene glycol 6000 till minimum volume, precipitated by acetone (1:1 v/v), the pellets were dissolved in PP buffer (pH 6.5, 10 mM)

Before trying peptides, he says he had been to his GP and was given physiotherapy sessions, but despite consistently doing the exercises he was told to do, it wasn't getting any better