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For immunoblotting, samples were resolved on 8%, 10%, or 12% polyacrylamide minigels (Bio-Rad) and transferred onto a PVDF membrane (GE Healthcare Life Sciences, PA) by wet or semi-dry transfer

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The focus on these specific strategies in research and therapeutic development highlights their potential to significantly enhance efficacy and outcomes

Increased oxidative phosphorylation activity often indicates cell activation and a change in function

Approximately 95% of the oxygen consumed is reduced to water during aerobic metabolism, but the remaining fraction may be converted to reactive oxygen species and other free radicals, inducing oxidative stress