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Site-directed mutagenesis Site-directed mutagenesis of the GST clone was performed using a QuikChange mutagenesis kit (Stratagene) according to the manufacturers protocol
To further support the impact of CBS on replenishing GSH levels under cystine-restricted conditions, we expressed a codon-optimized CBS (CBS OPT ), which is resistant to the designed sgRNA (Fig
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[16]Sana S, Ramzan M, et al
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Its sequence allows the peptide to remain relatively stable in certain biological environments compared to many other short peptides
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