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After incubation (10 min, 39C), the plates were centrifuged (200 x g, 5 min, 20C, Universal 320, Hettich, Tuttlingen, Germany) and the medium was replaced with pre-warmed XF Seahorse medium supplemented with glucose, sodium pyruvate and glutamine (all purchased from Agilent, Santa Clara, California, USA)
No, semaglutide does not directly affect sex drive through pharmacological mechanisms
The drug concentration in compounded vials is precise and skipping or reducing doses to ration medication causes loss of GI tolerance and weight regain
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