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Role of a dual glucose-dependent insulinotropic peptide (GIP)/Glucagon-like peptide-1 receptor agonist (twincretin) in glycemic control: from pathophysiology to treatment

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Sample preparation for T cell proteomics A375 melanoma tumor cells (ATCC, CRL-1619) were cultured in complete DMEM (Gibco, 11965-092) supplemented with 10% (v/v) FBS (VWR, 97068-085) and 1% penicillinstreptomycin (VWR, 16777-164) at 37 C under 5% CO 2

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Chaput N, Lepage P, Coutzac C, Soularue E, Le Roux K, et al

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Upon ligand/receptor interaction the intracellular portion of GLP-1/Notch is thought to translocate to the nucleus and interact with the LAG-1 transcription factor and the SEL-8/LAG-3 transcriptional co-activator (Lambie et al., 1991

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