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Evolution of procaspase-1-cleaving proteases Our initial campaign targeted cleavage within the interdomain linker (IDL) of procaspase-1

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Briefly, cells treated as above were added to 24-well plates and incubated for 20 min with DCFH-DA (10 mol/L) at 37 C

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This stress leads to suppression of forkhead box O1 (FoxO1) signaling and subsequent upregulation of thioredoxin interacting protein, inhibition of insulin and SOD-2 expression, re-expression of Neurog3, and -cell dedifferentiation and functional failure