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Diabetologia (1993) 36:7414

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Samples were fixed in 10% buffered formalin overnight at 4C, dehydrated with increasing concentrations of ethanol, embedded in paraffin, cut into 5 m sections, and stained with hematoxylin and eosin (HE) or Massons Trichrome Stain Kit (Sigma-Aldrich)

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However, you may be able to secure insurance coverage if your doctor justifies its use and/or youve tried but have not had success with other treatment options.