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These sequences were then run through an R script (Supplementary Materials, bash_DADA2 + IDTAXA_workflow_Illumina-PairedEnd_V1V3.R) which trimmed reverse reads to 260 bp, removed forward reads exceeding a maximum expected error of three, and truncated reverse reads exceeding a maximum expected error of six
The combination addresses fat metabolism from two directions
doi:10.1007/s12178-025-09990-7
It supports triglyceride hydrolysis during the measurement period but does not establish the final fate of released fatty acids or net long-term energy balance
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