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Following reduction, cysteines were alkylated by the addition of 20 mM chloroacetamide (400 mM) for 30 min at 37 C

The final cell density in the sample was adjusted to 0.2 10 cells per 1000 L by dilution with acetonitrile to achieve an acetonitrile/water ratio of 9:1 (v/v), as described above, ensuring symmetrical peak shapes in HILIC separations

However, this does not typically result in their death due to the capacity of Glu to synthesize glutathione (GSH), a vital antioxidant that plays a crucial role in cancer cells and their resistance to oxidative stress [45]

Flanagan JL, Simmons PA, Vehige J, Willcox MD, Garrett Q (2010) Role of carnitine in disease

Vitamin B12 deficiency is common in the UK, particularly among older adults