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Quantitative PCR RNA was extracted from brain or cell samples using TRIzol reagent, and its concentration was determined using a Nanodrop 2000 UVvis spectrophotometer

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In case of the TMABADH, a third variant (TMABADH.1) was tested here in addition to the versions TMABADH.1S (Kang et al., 2013) and TMABADH.2 (Franken et al., 2015)

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Device therapies such as intragastric balloon, endoscopic sleeve gastroplasty or vagal nerve blockade are generally less effective weight loss strategies than surgical intervention, but provide 1424% excess weight loss when used in the treatment of obesity [23]