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Cells were fixed with 4% paraformaldehyde (PFA) in phosphate-buffered saline (PBS) for 15 min at 37 C, quenched with 50 mM NH 4 Cl in PBS for 10 min at room temperature (r.t.), permeabilized with 0.1% Triton X-100 in PBS for 10 min at r.t., and blocked with 1% bovine serum albumin (BSA) in PBS for 10 min at r.t

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Johnson CC, Fordyce FM, Rayman MP (2010) Symposium on geographical and geological influences on nutrition: factors controlling the distribution of selenium in the environment and their impact on health and nutrition

Especially if you are taking any of the following: Anti-anxiety medicines like diazepam (Valium)