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The supernatant was loaded onto a 1 mL Nickel-Sepharose resin column and washed with 30 column volumes (CV) of washing buffer (50 mM TrisHCl pH 8.0, 300 mM NaCl and 20 mM imidazole)

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Pernicious anemia is a lifelong condition requiring ongoing B12 injections (typically monthly after the initial loading doses)

Although not directly supported, the above-mentioned study suggests that this rapid metabolic switch was mediated by the GCGR

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