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In assay of short-chain fatty acids (SCFAs), the fecal samples were reconstituted in 0.4 ml distilled water and the supernatant was acidified with a 1/5 volume of 50% H 2 SO 4 and 1/2 volume of dilution internal standard solution (50 g/ml), and then extracted with organic solvent ethyl ether
Although the time course of Nrf2 stabilization and nuclear translocation indicated a rapid increase with a peak at approximately 6 h for DEM, and a slow but gradual rise triggered by NFT, maximal accumulation of Nrf2 protein, as well as the induction of GCLC and glutathione synthesis, were almost identical for both NFT and DEM after incubation periods of and beyond 24 h (Fig
For example, in certain neurodegenerative diseases, modulating SIRT3 activity can help manage mitochondrial dysfunction and oxidative stress 182
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