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Bacteria were harvested via centrifugation at 1,000 g and resuspended in a buffer of 10 mM MgCl 2 at an OD 600 of 1.0

The process of cellular senescence involves multiple factors, including DNA damage, telomere shortening, and epigenetic changes, which lead to cell cycle arrest and ultimately result in cellular senescence (Zhang et al., 2024)

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doi: 10.1016/j.steroids.2016.01.021 200 AlayevASalamonRSBergerSMSchwartzNSCuestaRSnyderRBet al

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