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Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes
By detecting the appearance of the colored quinones on a TECAN infinite M200 absorption curves were recorded at different substrate molarities in a total volume of 200 l containing 50 mM sodium phosphate buffer at pH 6.0, 2 mM SDS and variable amounts of enzyme (Table S7) at 25 C
Step 1: Immediate Transfer
Ghrelin receptor activation and binding assays
Acta 1831 , 291299 (2013)
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