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The membranes were blocked in QuickBlock Blocking Buffer for 15 min (Beyotime) and then probed with primary antibodies against fibronectin, collagen I, GFAP, -SMA, TGF1, SMAD3, phospho-Smad3 (Thr8), SMAD4, -tubulin, and -actin (Supplementary Table 2)
& Bull, R
approved options with clear dosing will emerge Medical supervision is essential These potent compounds require qualified medical oversight Alternatives exist CagriSema and other evidence-based combinations offer proven approaches The rapid evolution of metabolic peptide research means today's experimental combinations may become tomorrow's standard therapiesor may prove ineffective or unsafe
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Fragpipe analysis All samples were analyzed using Fragpipe v24.0, MSFragger v4.1.1, IonQuant v1.11.20 and Python v3.11.11
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