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Next, we detected the levels of DCFH-DA and found that, compared to control cells, the ROS levels were significantly higher in ACTL6A-KD cells with or without H 2 O 2 treatment (Fig

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It seems that cells in/after oxygen restriction might alternatively use molecules with primary other functions such as neurotransmitters and/or alternative energy substrates

Oocyte maturation The selected oocytes were matured in medium containing M199 medium (Gibco by Life Technologies, USA) with 0.3% bovine serum albumin (BSA), 10 IU/mL pregnant mare serum gonadotrophin (PMSG) (Intergonan, Intervet Deutschland GmbH), 10 IU/mL human chorionic gonadotrophin (hCG) (Chorulon, Intervet International BV Boxmeer-Holland), and 50 g/mL gentamycin (Sigma-Aldrich, USA), supplemented with varying concentrations of GSH (0.0 mM, 0.5 mM, 1.0 mM, and 1.5 mM)

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