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It now seems that, in Arabidopsis, DHARs are not normally required for ascorbate recycling or to maintain ascorbate redox state even under conditions that are known to stimulate glutathione oxidation without affecting ascorbate levels (Rahantaniaina et al., 2017), contrary to previously reported findings in tobacco (Chen and Gallie, 2006
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Practical Considerations for Stacking Protocols in Research Models Once mechanism compatibility is established, practical protocol design becomes the critical variable
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