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They found that MYC amplification promotes transcription of the E3 ligase KLHL42, leading to pRB1 ubiquitination and degradation, thereby conferring resistance to CDK4/6 inhibitors

METHOD DETAILS Sample preparation FOXO4 FHD (95-195) was subcloned into the pET His6 Tobacco Etch Virus (TEV) LIC cloning vector (2B-T, obtained from Scott Gradia, Addgene Plasmid #29666) and transformed into BL21 (DE3) cells

Anti-inflammatory action: GHK-Cu suppresses pro-inflammatory cytokines including IL-6 and TNF-alpha, which are improved in aging tissues and contribute to tissue damage over time

L.PetrikD.et al

Keep it cool, dry, and shielded from direct light before use, refrigerating if holding it longer term