Briefly, whole Drosophila were fixed in 4% PFA for 12 h at 4 C, then rinsed 3 PBS for 3 h at RT with gentle shaking, and then immersed in FlyClear Solution-1 (8% THEED (2,2,2,2- (Ethylenedinitrilo)-tetraethanol) (Sigma-Aldrich), 5% Triton X-100 (Sigma-Aldrich) and 25% Urea (Fisher Scientific, in PBS) for 14 days at 37 C, as described previously 26
I decided to give it a try and Im glad I did
Approved Medical Uses: Prevention & Treatment of B12 Deficiency: Corrects low B12 due to poor nutrition, GI disorders, or malabsorption
This makes injections a powerful tool for quickly addressing a deficiency and its symptoms, helping you feel better, faster
Therapeutic Class: Active Ingredients: Vitamin B12, Sodium Selenite, Adenosine Triphosphate, Tetrasodium Dihydrate Salt, Potassium Aspartate Magnesium Aspartate Vitamin B12 + Minerals Injection is a supplement therapy used for the treatment of anemia in horses, cows, camels and sheep