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If you often feel tired despite sufficient rest, it might be worth considering your B12 levels

Sensitive and accurate: linear detection range 0.4 - 100 M in 96-well plate Simple and convenient: mix the DTNB reagent with sample, remove protein precipitates for proteinaceous samples, add a second reagent and read the optical density Low interference: amino acids and common buffers do not interfere Direct assays: reduced glutathione in whole blood, plasma, serum, urine, tissue and cell extracts Drug discovery/pharmacology: effects of drugs on glutathione metabolism Note: The below protocol is a sample protocol

Light and temperature fluctuations can degrade solutions

PMID: 90 RomeoA

Our previous publication details the brain tissue dissociation, neuronal sorting and profiling methods and the optimized Smart-Seq2 method 20