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The theory: replenishing intracellular glutathione during the follicle-maturation window may improve the oxidative environment that the developing egg sits in
Slides were also counterstained with mouse anti-insulin primary antibody (1:1000, 4 C overnight) and goat anti-mouse secondary antibody conjugated with Alex Fluor 594 (1:400, 1 h at room temperature)
8 Week Protocol
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