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Inhibition of SGLT2 reduces sodium reabsorption in the proximal tubule and enhances Na + delivery to the macula densa, promoting finally the TGF activation with consequent afferent arteriole vasoconstriction and reduction of the single-nephron-glomerular-filtration-rate ( Figure 1 )
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Consistent use over several months ensures the best chance for visible and lasting results
Twenty-four hours following seeding, transient transfections were performed using Lipofectamine 2000 (11668019, Invitrogen) according to the manufacturers instructions without including additional transformation carrier DNA
In this way, a much higher amount reaches the blood and tissues than with oral supplements
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