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Many may need to continue taking GLP-1 medications in order to maintain their weight loss and health goals The doses of medication may stay the same or may be reduced to find the lowest effective dose that maintains weight and health Lifestyle and behavioural changes in the maintenance phase are crucial Any reduction or stopping of GLP-1 medication should be done under medical supervision, gradually Monitor weight and appetite vigilantly Many people will find that they can use a reduced dose of GLP-1 medication once they have achieved their target BMI

But one unforeseen consequence of their popularity has been to create what is, in effect, one of the biggest off-label medical experiments ever

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Saxenda for weight management is dosed higher (3 mg) than Victoza for diabetes (1.21.8 mg)

Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates