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Higher NAD+ activates the sirtuin family of NAD+-dependent deacetylases, particularly SIRT1, which deacetylates PGC-1alpha (promoting mitochondrial biogenesis), FOXO transcription factors (enhancing stress resistance), and NF-kappaB (reducing inflammation)

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The only negative side effects the inoculated group reported were temporary injection site irritation and some mild fatigue

[6] Use on inflamed, infected, or wounded scalp areas may increase irritation risk and is typically deferred until integument integrity is restored

The GHK peptide, first isolated from human plasma, has been reported to participate in tissue-protective and regenerative processes, although definitive clinical efficacy in hair disorders remains under investigation