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the resin is cleaved with a cleaving agent and crude peptides are obtained by precipitating with diethyl ether, and crude peptides are obtained by RP-HPLC

Both the lyophilized powder and the reconstituted solution should be protected from direct light

Nonetheless, as discussed later, future research should validate the findings of the present single-biomarker model against multi-biomarker frameworks to confirm robustness under alternative structural assumptions

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