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General handling: Always replace the pen cap after each use to protect from light and maintain sterility
First-strand cDNA was synthesized using the Quantscript RT Kit (TianGen, #KR106) and used as a template for SYBR Green-based qPCR
Keep your vial in its original box or wrap it in aluminum foil if the box has been discarded
These unstable molecules are reactive and can damage other molecules, tissues, and cells
At this point further modifications catalyzed by aldehyde dehydrogenase and aldo-keto reductase allow to obtain carboxyethylmercapturic acid and 3-hydroxypropylmercapturic acid respectively, which are escreted with urine (Fig
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