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To confirm that the observed effects were mediated through direct GLP-1R activation, or indeed, that they are not secondary autocrine effects of insulin, we performed 2-NBDG uptake experiments in the presence of the GLP1R antagonist Exendin (939) and the potassium channel opener, diazoxide

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Essa abordagem metodolgica, centrada na pesquisa exploratria bibliogrfica, oferece uma base slida para a investigao aprofundada dos mecanismos de ao da semaglutida e suas contraindicaes na DM2, contribuindo para o avano do conhecimento em um tema de grande relevncia na rea da sade (CONTE, 2014)

For reducing SDS-PAGE, 2 g of protein was mixed with 3 L of 4 Bolt LDS Sample Buffer (Invitrogen), 1.2 L of 10 Bolt Sample Reducing Agent (Invitrogen), and Milli-Q water to a total volume of 12 L, and the samples were incubated for 10 min at 95 C