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Manual validation ensured accurate identification of all amino acid residues and post-translational modifications. Nuclear Magnetic Resonance (NMR) Spectroscopy To complement mass spectrometric data, ^1H and ^13C NMR spectroscopy were performed, providing insights into the conformational aspects and confirming the presence of specific functional groups, particularly in modified regions. Quality Control and Batch-to-Batch Comparability Assessment (New Section) Beyond single-batch characterization, peptide sequencing plays a vital role in establishing batch-to-batch consistency during late-stage development

Rushing or skipping steps leads to the problems that frustrate many users who then blame the peptide rather than their technique