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Western blotting Cells were harvested and suspended in Total Lysis Buffer (0.125 M Tris-HCl pH 6.8, 4% SDS and 20% glycerol)

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& Sobell, M

This sensation typically lasts only a short time and reflects the rapid cellular activity triggered by NAD

In the liver, although both increased FFA uptake and upregulated de novo lipogenesis contribute to the development of hepatic steatosis, accumulating evidence suggests that increased FFA uptake plays a predominant role in the development of fatty livers 1