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By middle age, production has dropped significantly

10.1016/j.heliyon.2023.e23750 58 LiY.ZhangT.SongQ.GaoD.LiY.JieH.et al (2023a)

[] Overproduction of ROS caused by exposure to heavy metals begins from cellular metabolism at the mitochondria and eventually reaches all cellular components and tissues which causes oxidative stress which then can result in the apoptosis of cells

Fractions corresponding to the front, middle, and back parts of the Ub4 peak were collected separately