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For interaction analysis with reduced roGFP2, GSSG was injected into the wells to a final concentration of 40 M

In such models, persistent corticosterone (CORT) treatment, exposure to erratic moderate stressors, and social defeat are standard techniques for producing depressive-like behaviors in animals

Note that acidic stock solutions should be diluted into buffered assay media before use to avoid pH artifacts in cell-based systems

This allows the peptide to work directly on the affected area, promoting faster recovery and healing

The whole protein was taken as the search space to include all potential interactions and the conformation with the lowest binding energy was selected