The more accurate concern is that rapid weight loss, reduced lean mass, and decreased mechanical loading may create bone health concerns in some populations, particularly when resistance training is absent
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Mice underwent fear conditioning over five consecutive days (days 15), during which freezing responsesquantified as the percentage of time spent freezingwere measured using Freezeframe 4 software (Actimetrics, IL, USA) to assess fear acquisition and consolidation
But in practice the scientists had to test out a number of different linker molecules, and chemically modify the GLP-1 structure slightly, in order to get the new drug to work
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