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Cell Culture The human colon cancer cell lines HCT116 and SW620, purchased from American Type Culture Collection (Manassas, VA, USA), were cultured in RPMI medium and DMEM/F12 (HyClone, Logan, UT, USA) respectively, supplemented with 10% foetal bovine serum (HyClone) and 1% penicillin and streptomycin
In the case of the 54-year-old man, the naturopath had prescribed high doses of vitamin D despite the man not having a history of vitamin D deficiency or bone loss
Starting with lower concentrations and increasing gradually can minimize this risk
However, the results could vary from person to person, depending on individual factors, skin condition, and genetic considerations
A transgenic HER-2/neu mouse study reported changes in mammary-tumour development
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