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For research designed to replicate or build on the existing literature, the 1:1 ratio is the proportion that connects your work to the existing evidence base

The coding sequences of genes obtained in the genetic screen were amplified from the pFL44L plasmids extracted from the library and subcloned into pRS426- TEF (2 ) plasmids using primers listed in Supplementary Table 2, which introduced Sma I and Xho I restriction sites

After 12 weeks of L-carnitine administration, serum ammonia levels were significantly lower in the carnitine group than in the control group (72.5 33 vs