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To account for autofluorescence, the MFI of the region of interest was corrected by subtracting the MFI of four background regions within the same image
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Specifically, accurate measurements were taken at precise intervals of 15, 30, 45 min, 1 h, 5 h, and one day after adding the working solution
This combination enables liposomes to be readily absorbed, allowing for systemic availability of the water-soluble materials that would otherwise not be absorbed into the body
