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For 1 H- 15 N heteronuclear single quantum coherence (HSQC) titration experiments, the concentration of p53 TAD PEP was increased while maintaining the same ratio of 14 N-FOXO4 FHD and 15 N labeled-p53 TAD
Common volumes are 1 to 3 mL
B12 is a soluble vitamin which can be deficient in some people due to illness or unhealthy eating
In general cyanocobalamin isn't retained as long as hydroxocobalamin so maintenance dosing tends to be more frequent - think in the states they tend to do loading doses as daily for one or two weeks followed by weekly for a few months and then monthly
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