The culprit might not be your protocol at all, but something far simpler: a dirt-cheap amino acid you're probably overloo
A typical assay consisted of 0.15 M Tris buffer, pH 8.0, 100 mM serine, 100 M NADH, and 10 L LDH of a 1:100 stock
This uncertainty is compounded by the fact that BPC-157 is often used without medical supervision , self-injected, and combined with other peptides or supplements
Singh AP, Singh M, Balakumar P
J Biol Chem 251 : 42874293 Pisoni RL, Acker TL, Lisowski KM, Lemons RM, Thoene JG 1990 A cysteine-specific lysosomal transport system provides a major route for the delivery of thiol to human fibroblast lysosomes: possible role in supporting lysosomal proteolysis