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doi 10.1016/j.nano.2016.08.004 84 YustaBBaggioLLKoehlerJHollandDCaoXPinnellLJet al

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Once in solution, refrigerate and keep sealed

The initial rapid and short-lived release facilitated by Large Dense Core Vesicles (LDCVs) at the plasma membrane is followed by a sustained release of insulin that depends on the mobilization of insulin-secreting granules (ISGs) to the plasma membrane for exocytosis 1