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The pressure in the column was maintained at 1800 psi and the samples were run for 30 minutes

Strikingly, our GSEA analysis also showed that ablation of Gclc in FoB displayed a similar increase in mTORC1 signaling genes compared to Gclc fl/fl FoB and Gclc fl/fl MZB (Fig

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After the last wash, cells were resuspended in DAPI mounting medium and seeded on Cell-Tak (Fisher Scientific 10317081) coated glass slides