The inconsistency between basic study and clinical practice may be explained by several underlying mechanisms: 1) epithelial injury during ARDS can limit the interaction between -agonists and its receptor, whereas continuous administration of -agonists downregulates the expression of receptors, thus leading to unresponsiveness
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The cells were cultured in DMEM (Gibco, Gaithersburg, USA) supplemented with 10% fetal bovine serum (Gibco, Gaithersburg, USA), 100 U/mL penicillin, and 100 g/mL streptomycin
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