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Caveolin-1 knockout mice exhibited decreased expression of ZO-1, occludin, and claudin-5 while showing increased MMP activity and enhanced BBB permeability following photothrombotic ischemia [103]

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Practical Application Breakfast: 25-30 grams (highest GLP-1 response) Lunch: 20-25 grams Dinner: 25-30 grams Eggs (6-7 grams per egg) Greek yogurt (15-20 grams per cup) Chicken breast (25 grams per 3 oz) Fish (salmon, cod, tuna: 20-25 grams per 3 oz) Legumes (15-18 grams per cup) Whey protein (20-25 grams per scoop) Strategic Timing: Consuming protein early in meals triggers earlier GLP-1 release, which may enhance satiety throughout the meal and reduce overall calorie intake

Understanding these microenvironmental barriers is essential to design precision biomaterial strategies such as hydrogels that can remodel the local milieu, optimize dose distribution, and enhance therapeutic efficacy

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