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While the IOM had based B12 needs on the amount required to correct hematological parameters for people with pernicious anemia, the EFSA had almost 20 more years of research to draw on, and concluded that the most suitable approach was basing needs on biomarkers (serum B12, holotranscobalamin, MMA, and homocysteine)
Many of these metal-based mimics effectively react with a wide range of free radicals, and their specificity for hydrogen peroxide is rather limited
-actin (mouse anti-human -actin, 1:5000, A5441, Sigma) was used as endogenous control
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