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Storage and handling Keep the vial securely closed when not in use

Quality control Four quality control (QC) samples were prepared by pooling 20 L of each sub-aliquoting corresponding sample into a new tube, and MCF derivatization was prepared in the aforementioned manner

Samples were run in duplicate following the manufacturers protocols, with the exception of longer sample and calibrator incubation for cytokine plates (overnight instead of 2 hours at 4C)

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