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TM3 cells were harvested after treatment, stained with Annexin V-FITC and PI reagent, and incubated in the dark for 15 minutes
This treatment successfully induced senescence, as evidenced by a significant increase in SA--gal activity
The B12 drip typically results in positive effects lasting one week
Many adults in Hawaii are low in B12 due to stress, diet, or absorption issues and injections are the most reliable way to correct it
This graduated escalation reduces the likelihood of side effects and gives the clinician time to assess patient response at each tier
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