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75 g of protein lysates were heated for 10 min at 95 C in Laemmli 6X SDS sample buffer (Boston BioProducts, # BP-111R) with 5% 2-mercaptoethanol (VWR Life Science #M131-100ml) and then ran on 420% Criterion TGX pre-cast gels (Bio-Rad #5671093)
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The assay diluent was then added to the plates and incubated for 1 h on a shaker to block nonspecific binding
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Fibroblasts multiply and develop in response to this deceiving signal, to make up for the loss of elastin in the skin
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