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In a randomized, double-blind trial, female volunteers applied GHK-Cu encapsulated in nano-lipid carrier twice daily for 8 weeks
Cells were washed three times with Krebs buffer (20 mM HEPES, 5 mM KH 2 PO 4 , 1 mM MgSO 4 , 1 mM CaCl 2 , 136 mM NaCl, 4.7 mM KCl, pH 7.4), then incubated with 500 g/ml 2-NBDG, a fluorescence-labeled deoxyglucose analog, for 20 min at room temperature
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doi: 10.1038/s41586-021-03207-w 66 NatarajanAZlitniSBrooksEFVanceSEDahlenAHedlinHet al
There were no significant differences in serotonin levels, and no associations were found between serotonin levels and sleep abnormalities or emotional rating scales
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