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Enzyme reaction was stopped with 1 M HCl and color development was measured at 450 nm on a microplate reader (FLUOstar OPTIMA, BMG Labtech)

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On the other hand, with ee change, a gradual change from blue to gray was observed (Fig

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Patients are strongly advised not to source retatrutide outside of a regulated clinical trial, in line with MHRA guidance on purchasing medicines online