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The protein sample was extracted from membrane by adding a 2 solubilization buffer containing 20 mM HEPES pH 7.5, 500 mM NaCl, 2% (w/v) n-dodecyl-beta-D-maltopyranoside (DDM, Affymetrix), 0.4% (w/v) cholesteryl hemisuccinate (CHS, Sigma), and 2% (w/v) glycerol for 3 h at 4 C
GIP stimulates also glucose-dependent insulin release in people without diabetes and may have a role in appetite regulation: despite mixed findings, preclinical research suggests that the addition of GIP RA may enhance appetite suppression and WL associated with a fixed-dose of GLP-1 RA (Fig
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Howard AD, Feighner SD, Cully DF, et al
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